Core Biocontrol Agent as Bionematicides

Bionematicides are biological pest control products derived from living microorganisms (bacteria, fungi), botanical extracts, or natural metabolites used to control plant-parasitic nematodes, such as root-knot nematodes and cyst nematodes. Unlike highly toxic chemical nematicides (e.g., carbofuran, fosthiazate), bionematicides offer an eco-friendly profile, leaving zero chemical residues and preserving soil ecology.


Core Biocontrol Agent as Bionematicides

Based on field efficacy and market adoption, commercial bionematicides are categorized into three primary groups:

🍄 Nematophagous and Egg-Parasitic Fungi (Long-Term Suppression)

These fungi manage nematode populations by trapping motile larvae with fungal mycelia or directly parasitizing nematode egg masses.

  • Purpureocillium lilacinum (formerly Paecilomyces lilacinus):
    • Mechanism: The most widely utilized “egg-parasitic fungus” globally. It secretes chitinases and proteases to penetrate nematode eggshells, destroying the eggs from within and breaking the reproductive cycle. It colonizes the rhizosphere effectively for sustained control.
    • Key Products: Melocon (Certis Biologicals), BioAct (Bayer).
  • Pochonia chlamydosporia (formerly Verticillium chlamydosporium):
    • Mechanism: An exceptional parasite of both cyst and root-knot nematode eggs. It reduces baseline soil infestation levels and provides preventive protection for high-value vegetables and tobacco.

🧫 Bacterial Nematicides (Dual Action: Knockdown & Root Promotion)

These beneficial bacteria colonize the root surface, creating a physical barrier while secreting metabolites or volatile compounds that repel or paralyze nematodes.

  • Bacillus firmus:
    • Mechanism: A premier bacterial nematicide. It disrupts egg hatching and induces paralysis in juvenile (J2) nematodes. Its hardy endospores tolerate drought and nutrient-poor soils, maintaining high viability under harsh conditions.
    • Key Products: Nortica (Bayer), Poncho Votivo (Bayer – primarily used as a commercial seed treatment for row crops).
  • Bacillus subtilis / Bacillus velezensis:
    • Mechanism: While working primarily as biofungicides and root growth stimulants, specific strains secrete lipopeptides that repel nematodes from root tissues. They are frequently used in soil-amendment blends.

Core Biocontrol Agents for Asian Soybean Rust

Asian Soybean Rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of the most destructive foliar diseases impacting global soybean production. Since this pathogen rapidly develops resistance to chemical fungicides, biological control methods utilizing beneficial microorganisms and their metabolites have become vital for sustainable disease management.


Core Biocontrol Agents (Microbial Biopesticides)

🟩 Bacillus Species (Bacterial Antagonists)

  • Bacillus subtilis: The most commercially mature bacterial solution. It competes aggressively for space and nutrients on the leaf surface and secretes lipopeptides (such as Surfactin) that disrupt rust urediniospores’ cell membranes. Field trials show that alternating B. subtilis with chemical fungicides reduces the ASR disease index by 41% to 69%.
  • Bacillus velezensis: Produces a broad spectrum of antifungal lipopeptides that strongly inhibit rust spore germination and germ tube elongation.

🟩 Metarhizium Species (Entomopathogenic & Antifungal Fungi)

  • Metarhizium anisopliae & Metarhizium humberi: Recent research highlights that their cell-free culture filtrates are rich in bioactive metabolites. These filtrates inhibit P. pachyrhizi urediniospore germination by 85% to 96%.
  • Dual-Action Protection: Applying Metarhizium reduces the fungal biomass of ASR within soybean leaves by roughly 30%, while simultaneously controlling major insect pests like soybean loopers and caterpillars.

🟩 Trichoderma Species (Hyperparasitic Fungi)

  • Trichoderma harzianum: Acts as a direct hyperparasite. It coils around and penetrates the rust pustules (uredinia), secreting chitinases and β-1,3-glucanases to degrade the rust cell walls while simultaneously triggering the plant’s Induced Systemic Resistance (ISR).

Field Application Methods of Metarhizium anisopliae

Metarhizium anisopliae is a broad-spectrum entomopathogenic fungus widely used in sustainable and organic agriculture for biological pest control.

Main applications in field:

Seed Dressing: Mixed with oil flowable concentrates to coat seeds (e.g., peanuts, sugarcane) for early-stage protection against grubs.

Soil Broadcast: Granular formulations are applied to the soil before sowing or during transplanting to build a long-term pest barrier.

Coarse Foliar Spray: Sprayed directly onto crops or the soil surface during peak pest activity or early larval stages.

Chemical-Biological Tank Mix: Combined with low doses of chemical insecticides or botanical pesticides (like matrine or azadirachtin) to accelerate pest knockdown and delay resistance.

Lentinan (LNT) / Shiitake Mushroom Polysaccharide

Category: Plant Immunomodulator & Bio-Antiviral Agent


Mechanism: It acts as an elicitor that stimulates the plant’s Systemic Acquired Resistance (SAR). It boosts defensive enzyme activities (SOD, POD, PAL) and strengthens plant cell walls to block infections, while directly inhibiting viral replication.


Applications: Primarily used to prevent and manage destructive plant viruses (e.g., Tobacco Mosaic Virus, Tomato Yellow Leaf Curl Virus) and suppress fungal/bacterial diseases like Powdery Mildew and Soft Rot. It also helps crops recover from herbicide injuries and climate stress.


Eco-Safety: 100% biodegradable and zero residue, making it a cornerstone product for organic farming.

Aminooligosaccharins (Chito-oligosaccharides / COS)

Category: Plant Elicitor, Immunomodulator & Bio-Antiviral Agent


Mechanism: It acts as a “plant vaccine.” Its molecular structure mimics fungal cell wall fragments, triggering the plant’s Systemic Acquired Resistance (SAR). It stimulates the production of defensive enzymes (chitinase, glucanase) and phytoalexins, while boosting endogenous growth hormones.


Key Uses: Prevents destructive crop viruses, controls stubborn fungal/bacterial diseases (Anthracnose, Powdery Mildew), suppresses root-knot nematodes, and crucially helps crops recover from extreme weather stress (frost, drought) and herbicide injuries.


Eco-Safety: Sourced from natural marine chitin (shrimp/crab shells), it is fully biodegradable, completely non-toxic, and a gold-standard input for certified organic farming.

Bacillus thuringiensis var. kurstaki (Btk)


Category: Microbial Stomach-Poison Insecticide (Biopesticide)


Mode of Action: Ingested by the target pest → Dissolved in the highly alkaline (pH >9.0) midgut of Lepidopteran larvae → δ-endotoxins bind to specific receptors and create pores in the cell membrane → Midgut perforation, leading to starvation and fatal septicemia within 48–72 hours.


Target Scope: Strictly targets Lepidopteran pests (caterpillars, borers, loopers, diamondback moths) in forestry and crops, while being 100% safe for humans, mammals, and beneficial predators like honeybees.


Application Tip: Must be applied against early-instar larvae (1st to 2nd instar) and during late afternoons or overcast days to prevent UV degradation of the crystal proteins.

Applications of Silicone Antifoams in Agriculture

In modern crop science, silicone antifoams (defoamers) serve as crucial “invisible engines” and high-efficiency agrochemical adjuvants. Agrochemicals often generate heavy foam during manufacturing, mixing, and spraying due to the high concentration of surfactants. This foam can drastically reduce efficacy and damage equipment.

Silicone antifoams solve these issues across four primary agricultural stages:

1. Agrochemical Formulation & Manufacturing (In-Can Adjuvants)

  • Suppressing Foam in High-Foam Formulations: Huge amounts of foam are generated by high-shear agitation when producing agrochemical formulations like Emulsions in Water (EW), Suspension Concentrates (SC), Wettable Powders (WP), and Soluble Powders (SP) . Silicone antifoams keep reactors operating safely and prevent overflow.
  • Enhancing System Stability: Due to their chemical inertness, silicone antifoams do not react with active ingredients like insecticides, fungicides, or herbicides. This ensures the long-term shelf life and stability of the product.

2. Bottling & Packaging Lines

  • Accelerating Filling Speed: High-speed automated filling lines can cause agrochemicals to foam up, leading to “false fullness” or spillages.
  • Ensuring Accurate Dosage: Eliminating foam ensures the net volume of each bottle is perfectly accurate, preventing under-filling and keeping the packaging clean.

3. Field Mixing & Tank-Mix Adjuvants

  • Resolving Field Dilution Foaming: When farmers dilute agrochemicals in water tanks or mix multiple pesticides with foliar fertilizers, vigorous agitation often causes foam to overflow, making it impossible to fill the tank to its correct capacity.
  • Instant Foam Knockdown: As a tank-mix adjuvant, adding a trace amount of silicone defoamer instantly collapses surface foam, allowing smooth, time-saving preparation.

4. Crop Spraying & Drone Aviation (Aviation Spraying)

  • Preventing Airlocks in Spray Equipment: In tractor boom sprayers or drone crop-dusting, air bubbles in the liquid cause nozzle spitting, dripping, or uneven spray pressure. Defoamers ensure stable line pressure and a uniform spray pattern.
  • Synergistic Performance (Spreading & Penetration): Many high-performance agricultural silicone defoamers also reduce surface tension. This helps the droplets spread rapidly into a uniform film on plant leaves (even those with thick waxy cuticles), increasing chemical retention, enhancing rainfastness, and maximizing pesticide utilization.

Rhamnolipids opportunities in Agricultural Biocontrol

Rhamnolipids are powerful, biodegradable biosurfactants that serve as a multi-functional “Green Shield” in modern sustainable agriculture. They combine direct pathogen elimination, pest control, and plant immunity boosting into a single eco-friendly solution.


Core Functions & Mechanism of Rhamnolipids

  • Direct Pathogen Destruction
    • Disrupts the cell membranes of phytopathogenic fungi.
    • Causes cell leakage and lysis of devastating crop diseases.
    • Effectively inhibits F. oxysporum (wilt) and B. cinerea (grey mould).
  • Broad-Spectrum Pest Control
    • Destroys the protective waxy cuticle of agricultural pests.
    • Leads to pest dehydration and targeted eradication.
    • Highly active against green peach aphids and mosquito larvae.
  • Plant Immunity Activation
    • Acts as a natural elicitor to trigger defense genes.
    • Stimulates defensive hormones like jasmonic and salicylic acid.
    • Equips crops (e.g., wheat, tobacco) with long-term systemic resistance.
  • Premium Bio-Adjuvant Performance
    • Reduces surface tension as an emulsifier and spreading agent.
    • Maximizes droplets adhesion and coverage on leaf surfaces.
    • Lowers the required dosage of traditional chemical pesticides.

Why Rhamnolipids Outperform Chemical types

  • Zero Residue: 100% biodegradable and eco-friendly to soil.
  • No Resistance: Physical membrane disruption prevents pathogen mutation.
  • Eco-Safety: Non-toxic to humans, livestock, and beneficial pollinators.
  • Multi-Value: Integrates disease cure, pest control, and crop priming.

Applications of Rhamnolipids

Rhamnolipids find widespread application across multiple major industries due to their unique combination of high surface activity, antimicrobial properties, and 100% environmental compatibility.

1. Household Cleaning & Personal Care

Rhamnolipids serve as direct, ultra-mild replacements for petroleum-derived surfactants like sodium lauryl sulfate (SLS).

  • Eco-detergents: They break down oils and grease effectively in laundry liquids and dishwashing soaps.
  • Skincare & Cosmetics: They act as non-irritating emulsifiers in creams, lotions, and baby care products.
  • Haircare: They generate dense, stable foam in shampoos while maintaining the skin’s natural moisture barrier.

2. Agriculture & Crop Protection

In farming, Rhamnolipids function as advanced biopesticides and biostimulants.

  • Antifungal Agents: They disrupt the cell membranes of plant pathogens like Phytophthora and Pythium, preventing root rot.
  • Biostimulation: They trigger the plant’s natural defense mechanisms, boosting immunity against pests.
  • Soil Health: They improve soil wetting capacity, helping roots absorb nutrients more efficiently.

3. Environmental Remediation

Rhamnolipids‘ ability to mobilize hydrophobic (water-repelling) substances makes them highly effective in environmental cleanup.

  • Oil Spill Cleanup: They safely disperse marine oil slicks without introducing toxic chemicals into aquatic ecosystems.
  • Soil Washing: They bind to and remove heavy metals (such as lead, cadmium, and copper) from contaminated industrial soil.
  • Biostimulation: They accelerate the ability of native soil bacteria to degrade hydrocarbons.

4. Oil Recovery & Heavy Industry

Rhamnolipids withstand extreme temperatures, high salinity, and fluctuating pH levels, making them ideal for harsh industrial environments.

  • Microbial Enhanced Oil Recovery (MEOR): They reduce interfacial tension inside oil reservoirs, releasing trapped crude oil from rock pores.
  • Metal Processing: They are used as eco-friendly lubricants, anti-corrosive coatings, and degreasers for industrial machinery.

5. Pharmaceuticals & Medicine

Rhamnolipids‘ biological activity opens doors for advanced medical applications.

  • Biofilm Disruption: They break down the protective biofilms formed by drug-resistant bacteria, making antibiotics more effective.
  • Drug Delivery: They form stable nano-micelles that can encapsulate and deliver hydrophobic medications directly to targeted tissues.
  • Antimicrobial Agents: They exhibit natural antiviral and antibacterial properties, useful in wound healing ointments.

Market Potential of PAECILOMYCES LILACINUS

Core Advantages and Mechanisms of PAECILOMYCES LILACINUS

  • Multiple Parasitism: As an endoparasitic fungus, it can parasitize nematode eggs and infect both larvae and adult females, effectively reducing the damage caused by root-knot nematodes and cyst nematodes.
  • Additional Benefits: In addition to its nematicidal activity, PAECILOMYCES LILACINUS can secrete substances such as indole acetic acid (IAA), which has the potential to promote crop root growth and increase yields.